spr SpectraMax iD5 SpectraTest ABS2 v1. Third this recommendation. EarlyTox Caspase-3/7 R110 Assay. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Figure 4: Transcreener ADP. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with abs. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. The M5 can read volumes down to 2uL with the. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. com 10. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 1. Published Dec 16, 2020 | Updated Dec 16, 2020. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. UV/Vis Photometric Performance: Wavelength Range. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. BMG Rubystar High. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. This process should take about 3 - 5 minutes. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Turn the reader on and secure the USB cable. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 FlexStation® 3 Multi-Mode Microplate Reader. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Luminescence was then measured using the microplate readers listed. One plate carrier adapter is. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Figure 1. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 1. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. . For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Supersedes 1. Learn how you can utilize microplate readers to drive landmark discoveries. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. SeePlateAdapteronpage20. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The following measurements are automatically made by SoftMax Pro Software: baseline noise, dark noise, optical alignment, wavelength accuracy, photometric linearity and wavelength precision. edu The content of this site is published by the site owner(s) and. no. 40266 Email: drugdiscoverytech@lifetech. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. SpectraMax M2, M2e;. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. com 4. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or We use Spectramax M5 microplate reader in our lab. ® reader to the computer. Data analysis Standard curves were plotted using the quadratic curve fit in SoftMax Pro Software, and the concentrations of cell lysates were calculated from the standard curves by the software. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. com 9. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Description The Onboard Microprocessor. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Microplate readers’ standard detection modes. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Plate chamber design of the SpectraMax category 1 microplate readers. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. The SpectraMax ® i3x. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. The reader has the most features and capabilities in one unit. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. book Page 1 Wednesday, July 7, 2010 2:09 PM Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. If the computer is connected to the Internet: Select Search Automatically for updated driver software. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 000000 out of 5. These robust readers have been placed in labs from. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 0 OD for earlier models. Published Dec 16, 2020 | Updated Dec 16, 2020. *This plate definition in SoftMax Pro Software is the same for black and white plates. D 3 1. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Then the plate was returned to the. SoftMax Pro. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. both cuvette-based and microplate. SpectraMax® M5 Multi-Mode Microplate Re. . Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Spectramax M5. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 305 reference level saturation. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Compatible Fluorescence Polarization Plate Readers. 7°C (gradient heating design). Plate readers can vary in attributes such as microwell plate format, throughput, optical system, wavelength range, and more. Figure 1. Where is the instrument serial number located? The serial. SpectraMax M5 reader SpectraMax M5e. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. D 1. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. With optimized reagents, option to operate the readers. Detection of pro-inflammatory factorsThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 0 out of 5 $ 5,999. 2. Multimode Reader Comparison Chart. 标准功. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Copia stocks a full line of used microplate readers. #3635 or Greiner cat. Choose from a three- (M3), four-. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Molecular Devices. Perform the scan and note the wavelength that gives maximum emission. i3x. . 9. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 VAL014, right table) assays on the SpectraMax M5, M5e, or FlexStation 3 reader are shown below. Catalog Number: M5. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. It was then read from the top and bottom on the SpectraMax iD3 reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. easy to use and read multiple plates very quickly. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 010 OD 384-Well Plate,. The instrument settings were identical to those used in Example 1 above. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. +44 118 944 8000. both cuvette-based and microplate reader applications SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A 5 1. Choose from a three- (M3), four- (M4), orThey include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Unplug the power cable from the back of the instrument. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Our absorbance plate readers feature our PathCheck Sensor. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orMolecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. Abs). SpectraMax M5e Multi-Mode Microplate Reader. The SpectraMax® iD3 Multi Mode Microplate . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. 87. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. optical beams than older wide-beam plate readers, are much more vulnerable to spurious readings due to dust. The performance of these two modes is not comparable to dedicated luminescence or timeresolved fluorescence instruments, or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. By offering two new configurations of multi-mode readers that deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an even greater combination of detection, application, and budgetary needs. The plate is set up for reading and images are acquired according to specified parameters. The plate reader. #655801. 8. Label: provides information. 95. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. PAINS analysisA single-mode plate reader is a good starting point. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. 3: The cuvette chamber. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader,. 2. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 316 grating motor driver faulty. Third this recommendation. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Dynamic Range. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. Choose from a three- (M3), four- (M4), orProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 3) At the bottom of the strip, click on "Show. Opens in a new window or tab. Select Update Driver. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. In the M2, M5 and Plus. Microplate Readers. The Analyst GT is equipped with 20 plate magazines in a bi-directional stacker. 1. Winnersh Triangle. 2. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. 40266 Email: drugdiscoverytech@lifetech. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. For more sample throughput, the system canThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Molecular devices SpectraMax M5 Pdf User Manuals. 9. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Molecular Devices Spectramax M5 Microplate Reader; Related products. 8. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 1. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. 8. D 1 1. HTRF is a. 000000 out of 5. Other technologies such as imaging. Add to Cart. The following document is. Fill a clean plate with water. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Please talk to your your local Tecan representative for the right configuration which suits your application demands. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. Most papers they cultured bacteria to OD600>0. 000 to 4. book Page 21 Wednesday, July 7, 2010 2:09 PM. The SpectraMax L is now connected to SoftMax Pro sofware. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 9000-0756. Maximum signal with lowest background. After the plate is read, data will appear in the plate section: 10. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. com. The plate was incubated at room temperature for one hour. Accordingly there are different configurations possible. Read More. Call 516-939-0888 to check. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Please refer to the Obsolete Microplate Reader Models for more information. A solution for low volume applications. Cells in each image are identified by SoftMax Pro Software and cell-by-cellit is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. This. I have been using the SpectraMax M5 since 2011. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. M5 E -HTRF Readers. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orThe Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). The optical systems . 314 low lamp level warning. 6 explains the lower values on the LmaxII384 plate reader. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Category 2 and 3 microplate readers use gradient heating as well. No additional manuals are available. SpectraTest ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. For more information please visit us at: M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. The chamber. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The reader temperature was set to 37 Celsius and most the the liquid in the wells of the plate had evaporated when I went to dispose of it. Most papers they cultured bacteria to OD600>0. Description 1. If it is something different, click on the arrow to bring up a list. Our absorbance plate readers feature our PathCheck Sensor technology and. 6% for the on-board optics (monochromators). SpectraMax® Microplate Readers and SoftMax® Pro Software. 0 . Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. SpectraMax M5 reader SpectraMax M5e. Standard curves were plotted using SoftMax® Pro Software (Figure 1). SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a. Prepare your iD series instrument for shipment. Bottom-reading is usually recommended for cell-based assay with adherent cells. In this product video, see how the SpectraMax M5 quickly reads samples. SpectraMax Glo Steady-Luc Reporter Assay Kit. We specialize in many of the Molecular Devices microplate readers, washers, and stackers including the SpectraMax®, VersaMax™, Gemini™, FlexStation™, AquaMax®, and StackMax® lines. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. Molecular Devices. 1. It features a modular design that allows users to add or change detection cartridges according to their needs. Opens in a new window or tab. e. SpectraTest ABS2 Absorbance Validation Package performance tests. Molecular Devices SpectraMax M5 Microplate Reader is available from The Lab World Group. 2. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 3) At the bottom of the strip, click on "Show. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. Molecular Device SpectraMax M2 Plate Reader - Certified with Warranty. A dialog box will appear, requesting you to confirm that the read is a pre-read. 0 out of 5 $ 15,999. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. There is currently no charge for using this plate reader. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Reader Documentation Version No. Application Area: Multi mode plate reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Only Molecular Devices microplate plate readers offer the capability to . 1. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 2. Description. Reader Documentation Version No. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Pleasanton, CA, USA. 1321N1, a human astrocytoma cell line, expresses. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 5 pM (0. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Shop now. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Cells in each image are identified by SoftMax Pro Software and cell-by-cell it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 11. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. 1. Fluorescence Microplate Reader Comparison Chart. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Recertification is recommended every two years. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. e. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. TR-FRET assay. 03 in x 3. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. 87. The advantage of using labeled probes is detection specificity. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspected. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Optics. The SpectraMax® ABS Plus Microplate Reader can run . 1. Choose from a three- (M3), four- (M4), or96-Well Plate, Normal Read Speed: 0. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Similar settings will work for other SpectraMax readers with fluorescence detection. 1) Select the correct plate and the wells that you want to measure. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSetup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 4. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 9000-0756. 0112-0115 F . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 2 Software (multiple versions available) Windows 7 PC 60 day warranty All necessary communication electrical cables The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. See our app note for details:. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. Shake. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. This reader's dual monochromators for. Supersedes 1. 000). Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 010 OD 2. The following document is intended. 8. Instructions. com 4. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and wester. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Here the delta mP was 336, and Z’ factor was 0. Plates were measured at 450 nm with 650 nm as a reference (Molecular Devices, SpectraMax M). When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. BMG Nephelostar Laser-Based Microplate Nephelometer Microplate Reader. edu, 814-863-3503.